Proteolytic Enzymes

Proteolytic Enzymes

A Practical Approach

eBook - 2001
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Book News
Beynon (veterinary preclinical sciences, U. of Liverpool, England) and Bond (biochemistry, molecular biology, Pennsylvania State U.) bring together chapters by specialists to cover many aspects of working with proteases, reflecting recent advances in the field. Topics include: the way enzymes are classified, purified, assayed and inhibited; the five classes of proteases and their mechanistic characteristics; proteinaceous inhibitors, their use in vivo , and the strategies for their identification; proteases as tools to study proteins using mass spectrometry; the use of proteinases for Edman sequence analysis and peptide marking; and protease inhibition and the prevention of proteolysis. Annotation c. Book News, Inc., Portland, OR (

Oxford University Press
Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The topics covered are: nomenclature and classification; purification; assay methods; determination of mechanism; inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing; limited proteolysis as a structural probe; synthetic function. This book will be as invaluable as the first edition in providing ideas and protocols for scientists either studying proteases or using proteases as a research tool.

Publisher: Oxford ; New York : Oxford University Press, 2001
Edition: 2nd ed
ISBN: 9780585483429
Characteristics: 1 online resource (xviii, 340 pages) : illustrations


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